COMBINATION OF PROTEIN FORMS FOR HORNFLY VACCINATION

摘要

Thrombostasin is an anti-clotting protein found in saliva of Haematobia irritans. Disclosed herein are studies testing blood uptake of horn flies feeding on cattle which confirm the association of ts genotype with blood uptake of horn flies. Blood uptake volumes of homozygous ts10 horn flies were lower than those of other ts genotypes when fed on control cattle. Cattle vaccinated with recombinant protein isoforms rTS9 or rTB8 resisted horn fly feeding by yielding lower blood volumes compared to flies feeding on control cattle. The impact of vaccination varied by ts genotype of flies. Cattle vaccinated with isoforms rTS9 resisted flies of ts2, ts9, and tb8 genotype. Vaccination with isoforms rTB8 produced resistance to ts8, ts9 and tb8 genotype flies. Horn flies of genotype ts10 were not affected with either TS isoforms and fed well on rTS9 and rTB9 vaccinated as on control-vaccinated cattle.

主权项

1. A vaccine composition comprising
a. an isolated thrombostasin peptide or protein selected from SEQ ID NO 1 or a variant thereof; SEQ ID NO 2 or a variant thereof; SEQ ID NO 3 or a variant thereof; SEQ ID NO 4 or a variant thereof; SEQ ID NO 5 or a variant thereof; and a combination thereof; and b. a pharmaceutically acceptable excipient.