HIV THERAPEUTICS AND METHODS OF MAKING AND USING SAME

摘要

HIV neutralizing peptides, sulfated HIV-1 envelope proteins and immunogenic fragments thereof are disclosed, as well as nucleic acids encoding these molecules and methods of producing these peptides, envelope proteins and fragments. Methods are also provided for the treatment or prevention of a human immunodeficiency type 1 (HIV-1) infection. The methods can include administering to a subject an HIV neutralizing peptide, sulfated HIV-1 envelope protein or immunogenic fragment thereof as disclosed herein. In several embodiments, administering the HIV neutralizing peptide, sulfated HIV-1 envelope protein or immunogenic fragment generates an immune response in a subject.

主权项

1. A method of making a sulfated HIV-1 envelope protein or immunogenic fragment thereof for use in stimulating an immune response against HIV-1 infection in a subject, the method comprising:
providing a plurality of HIV-1 envelope proteins or immunogenic fragments thereof comprising tyrosine residues at gp120 positions 173 or 177, or at both positions 173 and 177; sulfating the tyrosine residues at gp120 positions 173 or 177, or at both positions 173 and 177 on at least 90% of the HIV-1 envelope proteins or immunogenic fragments in the plurality of HIV-1 envelope proteins or immunogenic fragments; and purifying the plurality of HIV-1 envelope proteins or immunogenic fragments, thereby making the sulfated HIV-1 envelope protein or immunogenic fragment thereof for use in stimulating an immune response against HIV-1 infection in a subject.