| 摘要 |
PROBLEM TO BE SOLVED: To provide a method which can measure kinase activity with high sensitivity, to provide ATP derivatives and a reagent kit containing the same.SOLUTION: The invention provides a method for measurement of kinase activity comprising a step of reacting kinase, a substrate, and an ATP derivative containing a DNP group to introduce the DNP group into the substrate, a step of mixing a DNP group-containing substrate with an anti-DNP antibody, and a step of detecting a formed conjugate to measure kinase activity, wherein the ATP derivative is a compound in which a dinitrophenyl group is bound to a phosphate group of the γ-position of ATP through a linker; the ATP derivative; and a reagent kit containing the ATP derivative. |
