METHOD FOR ASSAYING CREATINE KINASE MB ISOZYME AND KIT TO BE USED THEREIN

摘要

The present invention addresses the problem of providing a method for specifically assaying CK-MB while avoiding the influence of CK-BB. Provided is a method for assaying CK-MB in a sample, said method comprising: a step for reacting the sample with a first antibody against CK-MB, a second antibody against CK-MB that recognizes a different epitope from the first antibody, and an anti-CK-B antibody that recognizes the 1st to 100th region from the N-end in the amino acid sequence of the B subunit of creatine kinase [step (1)]; a step for measuring an optical change that is caused by the aforesaid reaction [step (2)]; and a step for determining the quantity of CK-MB in the sample on the basis of the result obtained in step (2) [step (3)]. Also provided are: a kit to be used in the method; and a method for avoiding the influence of CK-BB in assaying CK-MB, said method being characterized by comprising treating a sample with the anti-CK-B antibody as described above.