| 摘要 |
Provided is the use of a TALE transcriptional repressor for modular construction of a synthetic gene line in a mammalian cell. Provided is a method for regulating expression of two proteins: an expression cassette A comprises: a feedback element coding sequence, a promoter A, a protein A and TALER protein A encoding gene, linked by means of a self-cleaving polypeptide, and a target sequence A comprising an shRNA1 target sequence; an expression cassette B comprises: a feedback element coding sequence, a promoter B, a protein B and TALER protein B encoding gene, linked by means of a self-cleaving polypeptide, and a target sequence B comprising an shRNA2 target sequence; an expression cassette C comprises a coding sequence consisting of a promoter and an activation element; a recombinant vector A having the expression cassette A, a recombinant vector B having the expression cassette B, and a recombinant vector C having the expression cassette C are introduced into a host cell; by means of the addition of shRNA1 or shRNA2, the expression of proteins A and B is regulated. |
