Microfluidic system with sample pre-treatment

摘要

Determining an analyte comprises (a) providing a microfluidic analytical device comprising at least a first, second and a third region, (b) obtaining a sample containing the analyte from the sample surface with a sampling element, (c) bringing the sampling element in the first region and eluting the analyte with an eluent, (d) transferring the obtained eluate in the second region and contacting with an analyte specific binding partner, (e) transferring the obtained mixture in the third region and contacting with a test element, and (f) determining the presence and/or quantity of analyte. Determining an analyte comprises (a) providing a microfluidic analytical device comprising at least a first, second and a third region, where the first region is in fluid communication with the second region and the second region is in fluid communication with the third region, (b) obtaining a sample containing the analyte from the sample surface with a sampling element, (c) bringing the sampling element in the first section of the microfluidic analytical device and eluting the analyte in the first region with an eluent, (d) transferring the eluate obtained in step (c) in the second region of the device and contacting the eluate with an analyte specific binding partner loaded in the second region for a period of at least 5 seconds, where a binding of the analyte to the analyte specific binding partner takes place, (e) transferring the mixture obtained in step (d) in the third region of the microfluidic analytical device and contacting the mixture with at least a test element, and (f) determining the presence and/or quantity of analyte in the test element. An independent claim is included for a kit for determining the analyte comprising: a microfluidic analytical device for determining the analyte comprising a first region for receiving the sampling element with ingested analytes and for eluting the analyte from the sampling element, a second region, which contains analyte specific binding partner and is in fluid communication with the first region, a third region, which comprises at least a test element for determining the presence and/or quantity of the analyte and is in fluid communication with the second region, optionally a fourth region, which contains an eluent to elute the analyte from the sampling element and is in fluid communication with the first region, and optionally a fifth region, which comprises at least a timer function for temporal monitoring of reaction running in the microfluidic analytical device, and optionally a housing; and a sampling element included in the microfluidic analytical device and optionally an eluent to elute the analyte from the sampling element.