发明名称 |
METHOD FOR QUANTIFYING MODIFIED PEPTIDES |
摘要 |
<p>The present invention provides a method for quantifying modified peptides in a sample, the method comprising: (a) obtaining peptides from the sample; (b) adding reference modified peptides to the peptides obtained in step (a) to produce a mixture of peptides and reference modified peptides; (c) carrying out mass spectrometry (MS) on said mixture of peptides and reference modified peptides to obtain data relating to the peptides in the sample; and (d) comparing the data relating to the peptides in the sample with data in a database of modified peptides using a computer program; wherein the database of modified peptides is compiled by a method comprising: (i) obtaining peptides from a sample; (ii) enriching modified peptides from the peptides obtained in step (i); (iii) carrying out liquid chromatography-tandem mass spectrometry (LC-MS/MS) on the enriched modified peptides obtained in step (ii); (iv) comparing the modified peptides detected in step (iii) to a known reference database in order to identify the modified peptides; and (v) compiling data relating to the modified peptides identified in step (iv) into a database.</p> |
申请公布号 |
EP2419739(B1) |
申请公布日期 |
2015.11.04 |
申请号 |
EP20100714354 |
申请日期 |
2010.04.16 |
申请人 |
HVIVO SERVICES LIMITED |
发明人 |
VANHAESEBROECK, BART;RODRIGUEZ CUTILLAS, PEDRO |
分类号 |
G01N33/68 |
主分类号 |
G01N33/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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