Oligopeptide sequence specifically bonding to phenylboronic acid group

摘要

An object of the present invention is to provide, as a method for immobilizing a protein molecule, protein immobilization method and means in which orientation of a molecule to be immobilized can be controlled, the molecule can be stably immobilized without a complicated process and a chemical group used for immobilization does not affect the activity and the function of the protein. Specifically, the present invention relates to a method for immobilizing a molecule including the steps of forming a labeled molecule by attaching a label peptide sequence comprising a hydroxyl group-containing amino acid to a molecule; and bringing a molecule having a phenylboronic acid group into contact with the labeled molecule, to capture the labeled molecule by the molecule having a phenylboronic acid group.

主权项

1. A method for immobilizing a molecule, comprising the steps of:
attaching a label peptide sequence comprising two or more amino acid residues of hydroxyl group-containing amino acids, and one to three amino acid residues selected from the group consisting of amino acid residues of basic amino acids and tryptophan residue to a molecule to form a labeled molecule; providing a molecule having a phenylboronic acid group with one or more functional groups bound to a solid support or crosslinked by a bridging molecule to form an immobilization substrate; and bringing the molecule having a phenylboronic acid group into contact with the labeled molecule, to bind and immobilize the labeled molecule by the molecule having a phenylboronic acid group.