Aperture biosensor with trenches

摘要

A luminescence sensor, comprising a non-transparent substrate structure (2) having at least one aperture (5) intended to comprise an analyte and a transparent substrate structure (3), which is arranged to or adjacent said first structure (2). The aperture has a smallest lateral dimension, which is smaller than half the effective wavelength of an excitation radiation, such as light at a wavelength of 700 nm, resulting in an effective wavelength in water of about 538 nm. The transparent structure has a trench (4) with a surface portion provided with ligands with an affinity towards a target molecule. The trench results in that a luminophore attached to the target molecule will be positioned at the entrance surface of the aperture, where the excitation energy is largest.

主权项

1. A sensor substrate, comprising:
a non-transparent substrate structure having at least one aperture for including analyte; a transparent substrate structure arranged adjacent to said non-transparent substrate structure; and at least one trench including a surface portion formed in the transparent substrate structure, the surface portion
coinciding with and forming a bottom of the at least one aperture,is arranged at a predetermined distance from said aperture, having a higher binding capacity in relation to said analyte than elsewhere on the transparent substrate, andcomprising a lateral in-plane dimension different than the corresponding lateral in-plane dimension of the aperture.