Compositions, kits and methods for detection of campylobacter nucleic acid

摘要

The disclosed invention is related to compositions, kits and methods comprising one or more oligomers targeting 16S rRNA target nucleic acid from Campylobacter species jejuni, coli and/or lari. Compositions include amplification oligomers, detection probe oligomers and/or target capture oligomers. Kits and methods comprise at least one of these oligomers.

主权项

1. A method for specifically detecting a Campylobacter target nucleic acid in a sample comprising the steps of:
(a) contacting a sample suspected of containing at least a Campylobacter target nucleic acid with at least two amplification oligomers that stably hybridize to a C. jejuni target nucleic acid and a C. coli, a C. lari, or a C. coli and a C. lari target nucleic acid, wherein a first of said amplification oligomers comprises SEQ ID NO:26 or SEQ ID NO:32 and wherein a second of said amplification oligomers comprises a target hybridizing sequence 15 to 45 nucleotides in length and configured to target a sequence in a region of a Campylobacter 16S rRNA gene corresponding to nucleotides 170 to 226 of GenBank Accession No.: AF393202.1, gi:20378208; (b) performing an in vitro nucleic acid amplification reaction wherein any of a C. jejuni target nucleic acid, a C. coli target nucleic acid and a C. lari target nucleic acid present in said sample is used as a template for generating an amplification product; and (c) performing a nucleic acid detection reaction that detects said amplification product to determine whether a Campylobacter target nucleic acid was present in said sample.