| 主权项 |
1. A non-naturally occurring microbial organism having nucleic acids encoding enzymes of a 1,4-butanediol (BDO) pathway, wherein said microbial organism is transformed with at least one heterologous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO, wherein said BDO pathway enzymes comprise:
(a) 4-aminobutyrate CoA transferase, said enzyme classified as EC 2.8.3.a and converting 4-aminobutyrate to 4-aminobutyryl-CoA; 4-aminobutyryl-CoA hydrolase, said enzyme classified as EC 3.1.2.a and converting 4-aminobutyrate to 4-aminobutyryl-CoA; or 4-aminobutyrate-CoA ligase, said enzyme classified as EC 6.2.1.a and converting 4-aminobutyrate to 4-aminobutyryl-CoA; (b) 4-aminobutyryl-CoA oxidoreductase (deaminating), said enzyme classified as EC1.4.1.a and converting 4-aminobutyryl-CoA to 4-oxobutyryl-CoA; or 4-aminobutyryl-CoA transaminase, said enzyme classified as EC 2.6.1.a and converting 4-aminobutyryl-CoA to 4-oxobutyryl-CoA; (c) 4-hydroxybutyryl-CoA dehydrogenase, said enzyme classified as EC 1.1.1.a and converting 4-oxobutyryl-CoA to 4-hydroxybutyryl-CoA, with the proviso that said non-naturally occurring microorganism is not a wild type microorganism inherently having any of the above genes; and (d) 4-hydroxybutyryl-CoA reductase (alcohol forming), said enzyme classified as EC1.1.1.c and converting 4-hydroxybutyryl-CoA to 1,4-butanediol; or 4-hydroxybutyryl-CoA reductase, said enzyme classified as EC 1.2.1.b and converting 4-hydroxybutyryl-CoA to 4-hydroxybutanal, and 1,4-butanediol dehydrogenase, said enzyme classified as EC 1.1.1.a and converting 4-hydroxybutanal to 1,4-butanediol. |
