Cell culture shaking device and shaking culture method as cell culture method

摘要

The invention intends to provide a cell culture shaking device which is able to improve the efficiency of cell culture while preventing the cells from becoming damaged. The shaking devices used in cell culture apparatuses for culturing cells in flexible culture bags for storing culture suspension having cells inoculated therein includes: shaking mechanisms having operating panels for pressing the culture bags repeatedly, so that the culture suspension in the culture bags is stirred by being pressed by a plurality of projections projecting for the operating panels.

主权项

1. A cell culture stirring device comprising:
a support member to support a flexible culture vessel which stores a culture suspension having cells inoculated therein; and a pressing unit comprising an operating plate having a surface that faces the support member to provide a space for receiving the culture vessel between the surface of the operating plate and the support member, wherein the pressing unit is configured to be movable upward and downward away from and toward the support member to press the flexible culture vessel, wherein the pressing unit has a plurality of projections which are positioned on the surface of the operating plate that faces the support member, and the plurality of projections have a smaller surface area than the surface of the operating plate, wherein the pressing unit is operable to press the culture vessel with the plurality of projections, wherein the pressing unit is capable of contacting and separating from the culture vessel, and wherein the pressing unit stirs the culture suspension in the culture vessel by pressing the culture vessel.