Synthesis of cleavable fluorescent nucleotides as reversible terminators for DNA sequencing by synthesis

摘要

This invention provides novel azido linkers for deoxynucleotide analogues having a detectable marker attached thereto.

主权项

1. A compound having a first, a second, and a third portion, wherein the second portion has the following structure:wherein α represents a point of attachment to the first portion and β represents a point of attachment to the third portion;
wherein the first portion comprises a deoxynucleotide or a dideoxynucleotide and the third portion comprises a detectable marker.