Compositions and methods including recombinant B lymphocyte cell line including an exogenously incorporated nucleic acid expressing an exogenous membrane immunoglobulin reactive to a first antigen and including an endogenous gene expressing an endogenous secreted immunoglobulin reactive to a second antigen

摘要

Compositions and methods are disclosed herein for producing one or more immunoglobulins in an isolated B lymphocyte cell line. An isolated cell line includes an isolated B lymphocyte cell line capable of expressing at least one exogenously incorporated membrane immunoglobulin reactive to a first antigen and at least one endogenous secreted immunoglobulin reactive to a second antigen.

主权项

1. An isolated recombinant cell line comprising:
an isolated B lymphocyte cell line including at least one exogenously incorporated nucleic acid, the isolated B lymphocyte cell line capable of expressing the at least one exogenously incorporated nucleic acid encoding at least one exogenously membrane immunoglobulin reactive to a first antigen that is a B lymphocyte-activating antigen and at least one endogenous gene encoding at least one endogenous secreted immunoglobulin reactive to a second antigen from a pathogenic agent.