Method for extracorporeal removal of a pathogenic microbe, an inflammatory cell or an inflammatory protein from blood

摘要

The present invention relates to a method for extracorporeal removal of a pathogenic microbe, an inflammatory cell or an inflammatory protein from mammalian blood/use of a device comprising a carbohydrate immobilized on a solid substrate, said carbohydrate having a binding affinity for a pathogenic microbe, an inflammatory cell or an inflammatory protein, for extracorporeal removal of said pathogenic microbe, inflammatory cell or inflammatory protein from mammalian blood/use of a carbohydrate having a binding affinity for a pathogenic microbe, an inflammatory cell or an inflammatory protein, wherein said carbohydrate is immobilized on a solid substrate, in the preparation of a device for treatment of a condition caused or aggravated by said pathogenic microbe, inflammatory cell or inflammatory protein/and a method for treatment of a mammalian subject suffering from a condition caused or aggravated by a pathogenic microbe, an inflammatory cell or an inflammatory protein.

主权项

1. A method for treating a subject in need thereof, by extracorporeal removal of Staphylococcus aureus, said method comprising:
a) contacting said subject's whole blood with heparin immobilized on a solid substrate, said heparin having a terminal residue, wherein heparin immobilization consists of a single covalent link of said terminal residue to said solid substrate by covalent end-point attachment, under conditions allowing binding of said Staphylococcus aureus in said subject's whole blood sample to the heparin; b) separating the whole blood from the solid substrate; c) recovering said whole blood containing a reduced amount of said Staphylococcus aureus; and d) reintroducing into said subject said whole blood containing a reduced amount of said Staphylococcus aureus.