Method for producing an L-amino acid using a bacterium of the family enterobacteriaceae having attenuated expression of the yjjK gene

摘要

The present invention provides a method for producing L-amino acids by fermentation using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to attenuate expression of the yjjK gene.

主权项

1. A method for producing an L-amino acid comprising:
(i) cultivating a bacterium belonging to the genus Escherichia in a culture medium to produce said L-amino acid in the culture medium, and (ii) collecting said L-amino acid from the culture medium, wherein said Escherichia bacterium has been modified to attenuate expression of a yjjK gene as compared with a corresponding non-modified Escherichia bacterium, wherein said modification to attenuate expression of said yjjK gene is by a method selected from the group consisting of inactivation of said yjjK gene on the chromosome of said Escherichia bacterium, modification of a region controlling expression of said yjjK gene on the chromosome of said Escherichia bacterium, and combinations thereof, wherein said yjjK gene comprises the nucleotide sequence of SEQ ID NO: 1 or a variant nucleotide sequence of SEQ ID NO: 1, wherein the variant nucleotide sequence is not less than 95% homologous to the nucleotide sequence of SEQ ID NO: 1.