| 摘要 |
Disclosed is a dimer comprising an isolated protein comprising a variant activin IIB receptor polypeptide (vActRIIB), wherein said polypeptide is selected from the group consisting of: (a) a polypeptide having the polypeptide sequence set forth in SEQ ID NO: 18, except for an amino acid substitution at position 28 with respect to SEQ ID NO: 18, wherein the substitution is a substitution of E with an amino acid selected from any one of A, F, Q, V, I, L, M, K, H, W and Y; (b) a polypeptide having the polypeptide sequence set forth in amino acids 19 through 134 of SEQ ID NO: 18, except for an amino acid substitution at position 28 with respect to SEQ ID NO: 18, wherein the substitution is a substitution of E with an amino acid selected from any one of A, F, Q, V, I, L, M, K, H, W and Y; (c) a polypeptide having the polypeptide sequence set forth in amino acids 23 through 134 of SEQ ID NO: 18, except for an amino acid substitution at position 28 with respect to SEQ ID NO: 18, wherein the substitution is a substitution of E with an amino acid selected from any one of A, F, Q, V, I, L, M, K, H, W and Y; (d) a polypeptide having the polypeptide sequence set forth in amino acids 25 through 134 of SEQ ID NO: 18, except for an amino acid substitution at position 28 with respect to SEQ ID NO: 18, wherein the substitution is a substitution of E with an amino acid selected from any one of A, F, Q, V, I, L, M, K, H, W and Y; and (e) a polypeptide having at least 90% identity to any one of (a) through (d), wherein the substitution at position 28 is a substitution of E with an amino acid selected from any one of A, F, Q, V, I, L, M, K, H, W and Y, and wherein the polypeptide is capable of binding myostatin, activin A, or GDF-11. Also disclosed is the use of the dimer in the manufacture of a medicament for inhibiting myostatin. |
