Anti-inflammatory compositions, methods and uses thereof

摘要

Disclosed is a method of testing the anti-inflammatory potential of a honey by the steps of: (a) stimulating a cell line with a suitable stimulant to induce an inflammatory response in the cells; (b) preparing a less than 10 kDa fraction from a honey by diluting the honey sample and collecting a less than 10kDa fraction by subjecting the diluted honey to a filtration separation step; (c) applying the filtered honey containing the less than 10 kDa fraction to the inflamed cells; (d) measuring the reduction in cytokine production and/or nitrogen oxide production to determine the anti-inflammatory potential of the honey sample wherein the greater the reduction in inflammation as measured via cytokine production and/or nitric oxide production, the greater the anti-inflammatory activity of the honey tested.