发明名称 |
LARGE COMMERCIAL SCALE LENTIVIRAL VECTOR PRODUCTION SYSTEM AND VECTORS PRODUCED THEREBY |
摘要 |
<p>In accordance with the present invention, a method for increasing the yield of rLV vector particles comprising a trans gene encoding a therapeutic protein or fragment thereof is disclosed. In one approach, cells are transfected with plasmids encoding the necessary components for rLV production using a calcium chloride transfection mix at pH 7.1 wherein the calcium chloride and plasmids form a complex which is added to the cells at a constant speed. The cells are then incubated for a suitable time period wherein virus particle media is collected at least twice during the incubation period and stored in a cold storage unit, thereby reducing virus inactivation.</p> |
申请公布号 |
HK1203360(A1) |
申请公布日期 |
2015.10.30 |
申请号 |
HK20150103829 |
申请日期 |
2015.04.21 |
申请人 |
THE CHILDRENS HOSPITAL OF PHILADELPHIA |
发明人 |
HIGH, KATHERINE, A.;WRIGHT, JOHN FRASER;HAUCK, BERND;QU, GUANG |
分类号 |
A61K |
主分类号 |
A61K |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|