PROCESS FOR THE PREPARATION OF AMOEBOCYTE LYSATE FROM THE HAEMOLYMPH OF THE HORSESHOE CRAB

摘要

THE PRESENT INVENTION RELATES TO A NON-DESTRUCTIVE PROCESS FOR THE PREPARATION OF AMOEBOCYTE LYSATE FROM THE HAEMOLYMPH OF HORSESHOE CRAB, CHARACTERIZED BY THE STEPS OF FIRSTLY, MEANS FOR ACCLIMATIZING A LIVE SPECIMEN OF HORSESHOE CRAB IN TREATED SEAWATER UNDER CONTROLLED CONDITIONS; THEN, MEANS FOR CLEANING THE LIVE SPECIMEN, INCLUDING CLEANING THE BASE OF THE LAST PAIR OF THORACIC APPENDAGES OF THE LIVE SPECIMEN; NEXT, MEANS FOR WITHDRAWING THE HAEMOLYMPH THROUGH THE BASE OF THE LAST PAIR OF THORACIC APPENDAGE UNDER ASEPTIC CONDITION; THEN, MEANS FOR TRANSFERRING THE COLLECTED HAEMOLYMPH INTO A PRE-COOLED DISPOSABLE CENTRIFUGE TUBE; THEN, MEANS FOR CENTRIFUGING THE COLLECTED HAEMOLYMPH TO SEPARATE AMOEBOCYTE CELLS FROM LYMPH; THEN, MEANS FOR DECANTING THE LYMPH OUT OF THE DISPOSABLE CENTRIFUGE TUBE; THEN, MEANS FOR WASHING THE WHITE AMOEBOCYTE CELLS AND CENTRIFUGING; THEN, MEANS FOR SONICATING THE AMOEBOCYTE CELLS FOR LYSING OF THE AMOEBOCYTE CELLS; THEN, MEANS FOR STORING THE SONICATED AMOEBOCYTE CELLS FOR FURTHER LYSING OF THE AMOEBOCYTE CELLS; THEN, MEANS FOR CENTRIFUGING THE SONICATED MIXTURE, THEREBY SEPARATING THE AMOEBOCYTE LYSATE IN THE FORM OF A SUPERNATANT; THEN, MEANS FOR DECANTING THE AMOEBOCYTE LYSATE INTO A STERILIZED VESSEL; THEN, MEANS FOR PRE-FREEZING THE AMOEBOCYTE LYSATE; AND SUBSEQUENTLY, FREEZE-DRYING THE AMOEBOCYTE LYSATE.