| 摘要 |
Discloses a process for detergent-free preparation of bacterial outer membrane vesicles (OMV) for use in vaccines, said process comprising the steps of: a) cultivating a population of a Gram-negative bacterium to stationary growth phase; b) at a time point at least about 1 hour after onset of stationary growth phase, incubating the bacteria obtained in a) in a medium adjusted to or having a pH higher than pH 8.0 and a concentration of a metal chelating agent of between about 1 and 100 mM to extract OMV; and, c) recovering the OMV extracted in b), wherein the recovery at least comprises removal of the bacteria from the OMV. Also discloses use of an OMV obtained by such a method to elicit immune reactions or treat meningitis. |
