| 发明名称 |
BACTERIAL ENGINEERING |
| 摘要 |
Described is a process for producing a mutant bacterium which exhibits improved survival and/or growth under a selected growth condition, the process comprising the steps of: (a) generating a pool of mutant bacteria by transposon mutagenesis with an activating transposon (TnA), wherein the TnA comprises a promoter capable of increasing transcription of a gene at or near its insertion site; (b) growing bacteria from the mutant pool under the selected growth condition and under one or more reference conditions to produce two or more test cultures; and (c) comparing the distribution of TnA insertions between test cultures to identify a first class of genes which are disadvantageous for growth and/or survival under the selected growth condition and a second class of genes which are advantageous for growth and/or survival under the selected growth condition. |
| 申请公布号 |
US2015307873(A1) |
申请公布日期 |
2015.10.29 |
| 申请号 |
US201514705958 |
申请日期 |
2015.05.06 |
| 申请人 |
Bactevo Ltd. |
发明人 |
Williams David Hugh;Turner Arthur Keith;Wain John Richard |
| 分类号 |
C12N15/10 |
主分类号 |
C12N15/10 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A process for producing a mutant bacterium which exhibits improved survival and/or growth under a selected growth condition, the process comprising the steps of:
(a) generating a pool of mutant bacteria by transposon mutagenesis with an activating transposon (TnA), wherein the TnA comprises a promoter capable of increasing transcription of a gene at or near its insertion site; (b) growing bacteria from the mutant pool under the selected growth condition and under one or more reference conditions to produce two or more test cultures; (c) comparing the distribution of TnA insertions between test cultures to identify a first class of genes which are disadvantageous for growth and/or survival under the and/or survival under the selected growth condition and a second class of genes which are advantageous for growth and/or survival under the selected growth condition; and (d) providing an engineered mutant bacterium in which at least one of said disadvantageous genes is removed or disrupted and/or at least one of said advantageous gene is overexpressed, such that the mutant bacterium exhibits improved survival and/or growth under the selected growth condition. |
| 地址 |
Cambridgeshire GB |
