CH3 DOMAIN VARIANT PAIR INDUCING FORMATION OF HETERODIMER OF HEAVY CHAIN CONSTANT REGION OF ANTIBODY AT HIGH EFFICIENCY, METHOD FOR PREPARING SAME, AND USE THEREOF

摘要

Disclosed are a CH3 domain variant pair of an antibody, a method for preparing same, and a use thereof. A mutation is induced in the CH3 domain so as to improve a yield of forming a heterodimer heavy chain constant region of an antibody. The CH3 domain heterodimer forms a heterodimer heavy chain constant region with a high efficiency of 90 to 95% or more and also has outstanding heat stability. A heterodimer heavy chain constant region including the CH3 domain heterodimer can construct a bispecific monoclonal antibody which simultaneously recognizes two kinds of antigens. The CH3 domain heterodimer and the bispecific antibody or fusion protein of an antibody constant region comprising same can be usefully applied to the treatment or prevention of a disease associated with a target antigen or a target protein.

主权项

1. A heterodimer comprising antibody CH3 domains, which comprises the following binding (a):
(a) in a first group of CH3 domain selected from the group consisting of Tyr349, Asp356, Glu357, Ser364, Lys370, Lys392, Asp399, Phe405 and Lys409, a binding between i) an amino acid selected from the group consisting of alanine (A), threonine (T), serine (S), valine (V), methionine (M), and glycine (G), which is substituted in at least one position of the first group, and ii) an amino acid selected from the group consisting of phenylalanine (F), tyrosine (Y) and tryptophan (W), which is substituted in at least one position of the first group (wherein, the positions are numbered according to the EU index).