| 主权项 |
1. A method for preparing multichaperone-antigen complexes comprising:
(a) contacting a biological sample with a solid phase to which HOP affinity molecules are covalently bound, under conditions such that multichaperone-antigen complexes in the biological sample bind said HOP affinity molecules, wherein the HOP affinity molecules comprise HOP TPR1, having an amino acid sequence as set forth in SEQ ID NO: 1, HOP TPR2a, having an amino acid sequence as set forth in SEQ ID NO: 2, HOP TPR1/2a, having an amino acid sequence as set forth in SEQ ID NO: 3, or a combination or variant of any one or more of the foregoing, wherein a variant is a HOP affinity molecule defined above containing deletions, insertions, substitutions or other modifications relative to the native HOP affinity molecule sequence and retains the specificity of the native HOP affinity molecule to bind heat shock proteins; (b) removing unbound components in the biological sample away from the solid phase; (c) eluting multichaperone-antigen complexes from the solid phase; and (d) recovering the eluted multichaperone-antigen complexes. |
