TWO-PHOTON PROBE FOR DETECTING COPPER(II) ION AND QUANTITATIVE ESTIMATION OF COPPER(II) ION IN HUMAN TISSUE USING THE SAME

摘要

The present disclosure relates to a two-photon probe for in-vivo imaging, having high selectivity for copper(II) ion, and a method for quantitatively estimating copper(II) on (Cu2+) in vivo using the same. The two-phoion probe according to the present disclosure can detect intracellular free copper(H) on for a long period of time with a penetration depth greater than 90 μm in living cells and tissues without the problems of mistargeting and photobleaching and can quantitatively estimate copper(II) on concentration in vivo using the ratio of blue emission intensity at 400-450 nm and red emission intensity at 550-650 nm.

主权项

1. A compound represented by [Chemical Formula 1]: wherein each of R1, R2 and R3, which are identical or different, is independently hydrogen or C1-C10 substituted or unsubstituted alkyl, X is —OCH2(CH2OCH2)nCH2OCH3, the substituted alkyl is substituted with a substituent selected from a group consisting of halogen, trifluoromethyl, amino, C1-C5 alkyl, C1-C5 alkoxy, hydroxyl, C1-C5 carboxyl, cyano, phenyl and benzyl, m is an integer from 1 to 3 and n is an integer from 1 to 6.