Compositions and methods are provided for modifying a rat genomic locus of interest using a large targeting vector (LTVEC) comprising various endogenous or exogenous nucleic acid sequences as described herein. Compositions and methods for generating a genetically modified rat comprising one or more targeted genetic modifications in their germline are also provided. Compositions and methods are provided which comprise a genetically modified rat or rat cell comprising a targeted genetic modification in the rat interleukin-2 receptor gamma locus, the rat ApoE locus, the rat Rag2 locus, the rat Rag1 locus and/or the rat Rag2/Rag1 locus. The various methods and compositions provided herein allows for these modified loci to be transmitted through the germline.
申请公布号
SG11201508028Q(A)
申请公布日期
2015.10.29
申请号
SG11201508028Q
申请日期
2014.04.16
申请人
REGENERON PHARMACEUTICALS, INC.
发明人
LEE, JEFFREY D.;MUJICA, ALEXANDER, O.;AUERBACH, WOJTEK;LAI, KA-MAN VENUS;VALENZUELA, DAVID M.;YANCOPOULOS, GEORGE D.