METHDOS FOR DETECTING ESTRADIOL BY MASS SPECTROMETRY

摘要

Provided are methods for determining the amount of estradiol in a sample using mass spectrometry. The methods generally involve ionizing estradiol in a sample and detecting and quantifying the amount of the ion to determine the amount of estradiol in the sample.

主权项

1. A method for determining the amount of estradiol in a test sample said method comprising:
(a) purifying estradiol from said test sample by liquid chromatography; (b) ionizing the purified estradiol from said test sample to produce one or more ions detectable by tandem mass spectrometry; and (c) detecting the amount of the estradiol ion(s) by tandem mass spectrometry, wherein the amount of the estradiol ion(s) is related to the amount of estradiol in said test sample; wherein the limit of quantitation of the method is less than or equal to 80 pg/mL and estradiol is not derivatized prior to mass spectrometry.