发明名称 |
ENGINEERED TRANSCRIPTION ACTIVATOR LIKE EFFECTOR (TALE) PROTEINS |
摘要 |
Disclosed herein are methods and compositions for design and use of engineered TALEs. |
申请公布号 |
US2015307561(A1) |
申请公布日期 |
2015.10.29 |
申请号 |
US201514695644 |
申请日期 |
2015.04.24 |
申请人 |
Sangamo BioSciences, Inc. |
发明人 |
Miller Jeffrey C.;Rebar Edward J. |
分类号 |
C07K14/195;C12N15/90 |
主分类号 |
C07K14/195 |
代理机构 |
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代理人 |
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主权项 |
1. An isolated cell comprising a target site and a non-naturally occurring TALE DNA-binding protein that binds to the target site, the TALE DNA-binding protein comprising a plurality of TALE-repeat units, each TALE-repeat unit comprising a repeat variable di-residue region (RVD), and wherein
(i) the RVD in the first repeat recognizes adenine (A); (ii) the target site does not comprise a 3′ adenine (A); (iii) the target site comprises 2 or fewer consecutive thymines (Ts); and (iv) the target site does not contain 5′-GG-3′ or 5′-GC-3′ dinucleotides. |
地址 |
Richmond CA US |