METHOD AND TOOLS FOR THE DETERMINATION OF CONFORMATION AND CONFORMATIONAL CHANGES OF PROTEINS AND OF DERIVATIVES THEREOF

摘要

A Method for the detection of the conformational state of a protein contained in a complex mixture of further proteins and/or other biomolecules, is proposed as well as assays for such a method. The method comprises the following steps: (1) Limited proteolysis of the complex mixture under a condition where the protein is in the conformational state to be detected leading to a first fragment sample; (2) Denaturation of the first fragment sample to a denaturated first fragment sample; (3) Complete fragmentation of the denaturated first fragment sample in a digestion step to a completely fragmented sample; and (4) Analytical analysis of the completely fragmented sample for the determination of fragments characteristic of having been the result both the limited proteolysis of step 1 as well as of the complete fragmentation in the digestion step 3. for the determination of the conformational state.

主权项

1. A method for the detection of the conformational state of a protein contained in a complex mixture of further proteins, other biomolecules, or both, comprising, the following steps:
1. Limited proteolysis of the complex mixture under a condition where the protein is in the conformational state to be detected leading to a first fragment sample; 2. Denaturation of the first fragment sample to a denaturated first fragment sample; 3. Complete fragmentation of the denaturated first fragment sample in a digestion step to a completely fragmented sample; 4. Analytical analysis of the completely fragmented sample for the determination of fragments characteristic of having been the result both the limited proteolysis of step 1. as well as of the complete fragmentation in the digestion step 3. for the determination of the conformational state.