METHODS AND COMPOSITIONS FOR SCREENING AND DETECTING BIOMARKERS

摘要

The present invention is concerned with a novel antigens associated with human tumors, including carcinomas of the colon or lung, as well as novel monoclonal antibodies which specifically bind to said antigen. The antibodies bind to normal human cells to a much lesser degree than to tumor cells. The antigens include 100 kDa glycoprotein that has a colorectal cancer membrane bound and a soluble form, has a UV absorbance peak at about 228 nm, an isoelectric point of about 3.5 to 4, a sialic acid content of about 20%, and does not substantially bind to an antibody specific for ACT. The antibodies find use both in methods such as the detection of malignant cells associated with tumors and in monitoring therapeutic treatment of humans with tumors.

主权项

1. An immunoassay for determining whether a subject is at risk of colorectal cancer comprising:
a) combining an antibody or antigen binding fragment thereof with a human tissue or fluid sample from the subject; wherein the antibody or antigen binding fragment specifically binds to a 100 kDa glycoprotein comprising SEQ ID NO:1, but does not substantially bind to glycosylated 180 kDa CEA and one or more linear peptides consisting of a linear peptide of 15 amino acids from amino acids 1-60 of SEQ ID NO:2, a linear peptide of amino acids 111 to 125 of SEQ ID NO:3, and/or a linear peptide of 15 amino acids from amino acids 150-701 of SEQ ID NO:4; and b) determining the amount of the antigen in the human tissue or fluid by determining the amount of the antibody antigen complex.