| 摘要 |
The invention relates to methods of optically screening compounds for their effects on neuronal cells expressing optogenetic proteins that initiate and report electrical activity in cells using light. Thus the invention provides high-capacity methods for primary screening of, for example, randomized chemical libraries. These high-throughput assays provide robust electrophysiological measurements of cells without requiring patch clamp techniques. Since the described optogenetic constructs and pluripotent stem cell (PSC)-derived cells operate to provide the precision, temporal resolution, and voltage control required for monitoring effects of compounds, assays of the invention are compatible with primary screening and drug discovery. Methods of the invention may find application in studying or addressing autism, epilepsy, Alzheimer's, amyotrophic lateral sclerosis, tuberous sclerosis, and ion channel modulators. |
