ANTI-CAMPYLOBACTER JEJUNI ANTIBODIES AND USES THEREFOR

摘要

Campylobacter jejuni is a leading cause of bacterial food-borne diseases in humans, ranging from acute diarrheal disease to neurological disorders. An isolated or purified antibody or fragment thereof specific to C. jejuni is described. The antibody or fragment thereof binds to a flagellar protein and reduces motility of C. jejuni. The antibody or fragment thereof is derived from a heavy chain IgG variable domain fragment (VHH) of a camelid animal immunized with C. jejuni flagellar protein. A multivalent form, as well as a phage format, of the antibody or fragment thereof is described. Methods of reducing presence of C. jejuni in an animal or an animal environment, methods and formulations for treating C. jejuni infection, and method of detecting C. jejuni are also described.

主权项

1. An isolated or purified antibody or fragment thereof specifically binding to C. jejuni flagella, comprising
a complementarity determining region (CDR) 1 comprising the sequence GLTFRNFHMA (SEQ ID NO:1) or VSTFSINALG (SEQ ID NO:4); a CDR2 comprising the sequence ISWSRDRQ (SEQ ID NO:2) or IGSDGTV (SEQ ID NO:5); and a CDR3 comprising the sequence AARTASASGDVVYKGSYQY (SEQ ID NO:3) or NAAGKRIGSDGSIWFAVASFGS (SEQ ID NO:6),wherein the isolated or purified antibody or fragment thereof specifically binds to flagellin.