发明名称 |
MATURATION OF HEPATOCYTE-LIKE CELLS DERIVED FROM HUMAN PLURIPOTENT STEM CELLS |
摘要 |
The present invention relates to directed differentiation and maturation of hepatocyte-like cells. In particular, the present invention relates to exposure of hepatocyte-like cells to an activator of a retinoic acid responsive receptor, such as retinoic acid (RA), optionally in combination with an inhibitor of GSK-3 (Glycogen synthase kinase 3) or activator of Wnt signalling and/or with the overlay of the cells with one or more components characteristic of the mammalian extracellular matrix (matrix overlay). The present invention also relates to exposure of hepatocyte-like cells to an activator of a retinoic acid responsive receptor, such as retinoic acid (RA), optionally in combination with an inhibitor of a cycline dependent kinase (CDK) and/or with the overlay of the cells with one or more components characteristic of the mammalian extracellular matrix (matrix overlay). The hepatocyte-like cells obtained in accordance with the present invention show a phenotype which is more similar to that of primary hepatocytes than previously shown. |
申请公布号 |
US2015307839(A1) |
申请公布日期 |
2015.10.29 |
申请号 |
US201314648365 |
申请日期 |
2013.11.28 |
申请人 |
TAKARA BIO EUROPE AB |
发明人 |
Küppers-Munther Barbara;Edsbagge Josefina |
分类号 |
C12N5/071 |
主分类号 |
C12N5/071 |
代理机构 |
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代理人 |
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主权项 |
1. A method for promoting the maturation of human hepatocyte-like cells, the method comprising:
Exposing said human hepatocyte-like cells to an activator of a retinoic acid responsive receptor. |
地址 |
Goteborg SE |