ANTIBODY-SN-38 IMMUNOCONJUGATES WITH A CL2A LINKER

摘要

The present invention concerns improved methods and compositions for preparing SN-38 conjugates of proteins or peptides, preferably immunoconjugates of antibodies or antigen-binding antibody fragments. More preferably, the SN-38 is attached to the antibody or antibody fragment using a CL2A linker, with 1-12, more preferably 6 or less, most preferably 1-5 SN-38 moieties per antibody or antibody fragment. Most preferably, the immunoconjugate is prepared in large scale batches, with various modifications to the reaction scheme to optimize yield and recovery in large scale. Other embodiments concern optimized dosages and/or schedules of administration of immunoconjugate to maximize efficacy for disease treatment and minimize side effects of administration.

主权项

1. A method of delivering SN-38 to a target cell comprising:
a) producing a compound, CL2A-SN-38, of the structure, by a reaction scheme as shown: wherein the reaction scheme further comprises reacting SN-38 with tert-butyldimethylsilyl chloride (TBDMS-C1) to produce 10-O-TBDMS-SN-38 (intermediate 4), wherein the reaction is carried out in dichloromethane solvent; b) attaching an antibody or antigen-binding antibody fragment to the CL2A-SN-38 to produce a mAb-CL2A-SN-38; and c) administering the mAb-CL2A-SN-38 to a subject.