MULTIPLE TARGETED RNAI FOR THE TREATMENT OF CANCERS

摘要

The present invention includes compositions and methods for making and using a RNAi capable of reducing expression of two or more genes, comprising: a first RNAi molecule that reduces the expression of a first target gene; a second RNAi molecule that reduces the expression of the first or a second target gene; and optionally a third RNAi molecule that reduces the expression of the first, the second, or a third target gene, wherein the RNAi molecules reduce the expression level of, e.g., mutated KRAS, SRC-3, EGFR, PIK3, NCOA3, or ERalpha1, and can be, e.g., miRNAs, shRNAs, or bifunctional shRNAs.

主权项

1. A bifunctional shRNA composition capable of reducing expression of three or more genes, comprising:
a first bifunctional RNA molecule that reduces the expression of a first gene target; a second bifunctional RNA molecule that reduces the expression of a second gene target; and a third bifunctional RNA molecule that reduces the expression of a third gene target, wherein each of the bifunctional RNA molecules are capable of activating a cleavage-dependent and a cleavage-independent RNA-induced silencing complex for reducing the expression level of the first, second and third gene target.