Closed system separation of adherent bone marrow stem cells for regenerative medicine applications

摘要

A method for isolating and processing bone marrow derived stem cells, including the steps of: (a) collecting a biological sample containing adherent bone marrow stem cells in a receptacle with interior walls coated with a cell-adherent substrate; (b) incubating the bone marrow cells on the adherent substrate so that a layer of adherent bone marrow stem cells adheres to the substrate; (c) washing any non-adherent cells from the substrate; and (d) collecting the bone marrow stem cell layer. Isolation kits and use of bone marrow cells harvested for cell therapies are also described.

主权项

1. A stem cell isolation kit comprising:
a) a first bag containing a plasma blood expander; b) a second bag containing an anticoagulant and a growth medium, said second bag comprising an injection port for introduction of a biological sample comprising red blood cells and stem cells; c) a third bag for collection of sedimented red blood cells from the second bag; d) a fourth bag comprising an interior surface coated with a cell-adherent surface layer for collection and isolation of adherent stem cells; e) a fifth bag containing a preservative for storage of adherent stem cells; wherein said first and second bags are interconnected with each other through sterile tubing for transferring the contents of the first bag to the second bag; the second and third bags are interconnected with each other through sterile tubing for sedimenting red blood cells from the second bag to the third bag; the second and fourth bags are interconnected with each other through sterile tubing for transferring of stem cells from the second bag to the fourth bag; and wherein the fourth and fifth bags are interconnected with each other through sterile tubing for the transfer of adherent stem cells from the fourth to the fifth bag.