发明名称 |
método para produzir uma glicosidade mutante, composição de enzimas para sacarificação de bikomassa e método para hidrolisar biomassa |
摘要 |
A glucosidase having improved cellulose degradation efficiency is provided and an efficient method for hydrolyzing biomass using the glucosidase is provided. The present invention provides a method for producing a mutant glucosidase, which includes introducing DNA encoding a secretion signal sequence and DNA encoding Asn-X-Ser or Asn-X-Thr into DNA encoding a glucosidase derived from a thermophile, and introducing the resulting DNA into a eukaryotic microorganism and expressing it as a secretory protein. The present invention also provides an enzyme composition containing the mutant glucosidase and a method for hydrolyzing biomass using the enzyme composition. |
申请公布号 |
BR112012021230(A2) |
申请公布日期 |
2015.10.27 |
申请号 |
BR20121121230 |
申请日期 |
2011.01.26 |
申请人 |
TORAY INDUSTRIES |
发明人 |
HIROYUKI KURIHARA;KATSUSHIGE YAMADA;KAZUHIKO ISHIKAWA;SHIOMI WATANABE;YASUNOBU WADA;YUJI KADO |
分类号 |
C12N1/19;C12N1/20;C12N9/42;C12N15/09;C12P19/02 |
主分类号 |
C12N1/19 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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