Microfluidic device-based nucleic acid purification method

摘要

A method is provided for purifying nucleic acid from a sample in a microfluidic device. The method can be used to purify nucleic acids from any source known in the art that comprises nucleic acids, such as prokaryotic or eukaryotic organisms, viruses, cell, tissues, organs, etc. In a specific example, the tissue is whole blood. The method for purifying nucleic acid may run fully automated in the microfluidic device.

主权项

1. A microfluidic device-based method for purifying nucleic acid from a lysed liquid sample solution, comprising:
providing a suitable microfluidic device comprising a first purification matrix and a second purification matrix; performing a first purification step using the first purification matrix, the first purification step comprising the steps of:
adding an organic solvent to the lysed solution;adding a first capture medium to the lysed solution;capturing the nucleic acid to the first capture medium;separating the nucleic acid-bound first capture medium from the solution;washing the nucleic acid-bound first capture medium with a wash solution;separating the washed first capture medium from the wash solution; andeluting the nucleic acid from the washed first capture medium; performing a second purification step using the second purification matrix, the second purification step comprising the steps of:
reestablishing an appropriate molecular charge on the eluted nucleic acid;re-capturing the nucleic acid from the solution using a second capture medium;washing the re-captured nucleic acid with a wash solution; andeluting the re-captured nucleic acid to obtain a purified nucleic acid.