Procedimiento de doble inclusión para el estudio histológico y/o de localización de marcadores moleculares en especímenes pluricelulares de dimensiones microscópicas

摘要

The invention relates to a method involving the double inclusion of pluricellular specimens of microscopic dimensions (70-300μιη), which allows the three-dimensional spatial arrangement of the samples to be maintained in order to subsequently carry out histological, immunohistochemical and/or localisation studies on molecular markers. The method includes preparing the sample by means of washes with a buffer solution; fixing the sample for 10-15 minutes at a temperature of between 2 and 8°C; dehydrating the sample with increasing concentrations of ethanol for 10-15 minutes respectively, at a temperature of between 2 and 8°C; and pre-inclusion in agar and inclusion in paraffin, without centrifuging the sample in any of the steps of the method. The invention also includes a kit with the elements necessary for the implementation of the method described in the invention.