FLUOROIMMUNOASSAY METHOD USING POLYPEPTIDE COMPLEX CONTAINING FLUOROLABELED ANTIBODY VARIABLE REGION

摘要

This invention addresses the problem of providing an immunoassay method that does not require a solid phase immobilization step or a washing step makes it possible to quantitatively measure the target substance in liquid phase in a speedy and simple manner makes it possible to visualize antigens and has high detection sensitivity. This problem is resolved by: sequentially performing (a) a step for bringing in liquid phase into contact with antigens in the measurement sample a complex comprising a polypeptide containing an antibody heavy chain variable region and a polypeptide containing an antibody light chain variable region in which the polypeptide containing an antibody heavy chain variable region and/or the polypeptide containing an antibody light chain variable region is labeled by a fluorochrome (b) a step for detecting the fluorescence of the fluorochrome or measuring the fluorescence intensity and (c) a step for calculating the amount of antigens contained in the test subject or visualizing the antigens using as an indicator the fact that the antigen concentration and the fluorescence intensity of the fluorochrome are positively correlated; and measuring the concentration of the target antigen present in the tested substance.