| 主权项 |
1. A method for detecting a pathogen in coldwater fish, comprising:
providing a sample potentially containing one or more nucleotide sequences of a pathogen in a coldwater fish; providing an oligonucleotide primer pair defining the 5′ ends of two complementary strands of a double stranded target sequence on the one or more nucleotide sequences of the pathogen; providing a polymerase; blending the sample, the oligonucleotide primer pair, the polymerase, deoxyadenosine triphosphates (dATPs), deoxycytidine triphosphates (dCTPs), deoxyguanosine triphosphates (dGTPs), and deoxythymidine triphosphates (dTTPs) in a container to form a polymerase chain reaction (PCR) mixture; subjecting the PCR mixture to insulated isothermal polymerase chain reaction (iiPCR) by heating the bottom of the container at a fixed temperature to form a PCR product; and detecting the PCR product to identify the double stranded target sequence. |
