| 发明名称 |
IMPROVED CULTIVATION MEDIA AND PROCESS FOR IMPROVED PROTEIN PRODUCTION BY PICHIA STRAINS |
| 摘要 |
The present invention provides optimized cell culture media and fed-batch cultivation processes to improve the viability and volumetric production of heterologous proteins in Pichia. The disclosed media and processes utilize a non-fermentable sugar or sugar alcohol as an osmoprotectant to improve the robustness of Pichia production strains during methanol inducible fermentation. |
| 申请公布号 |
US2015299648(A1) |
申请公布日期 |
2015.10.22 |
| 申请号 |
US201314647874 |
申请日期 |
2013.11.22 |
| 申请人 |
MERCK SHARP & DOHME CORP. |
发明人 |
Kim Sehoon;D'Anjou Marc;Mallem Muralidhar R.;Shandil Ishaan;Nylen Adam;Sharkey Nathan;Shaikh Seemab S. |
| 分类号 |
C12N1/16;C07K16/00;C07K14/62 |
主分类号 |
C12N1/16 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A cell culture medium for the production of a protein of interest in yeast host cells using a fed-batch fermentation process wherein the cell culture medium comprises a basal medium supplemented with an osmoprotectant. |
| 地址 |
Rahway NJ US |
