Peptide MHCII Tetramers to Detect Endogenous Calnexin Specific CD4 T Cells

摘要

The present application discloses proteins or peptides and methods of using such proteins or peptides to evaluate the immune status of a patient. In one embodiment, proteins or peptides may be used to detect endogenous calnexin specific CD4 T cells. In one preferred embodiment, the proteins or peptides may comprise peptide-MHCII tetramers (pMHC tetramers).

主权项

1. A method for evaluating the immune status of a patient against a fungus, the method comprising the steps of:
a) obtaining peptide-MHCII tetramers; b) exposing a patient's sample to a suitable amount of the pMHCII tetramers; c) identifying helper T cells in the patient's sample; d) quantifying the helper T cells in the patient's sample; and e) monitoring the response, expansion and characteristics of the helper T cells the after infection and vaccination, wherein the immune status of a patient against the fungus is obtained by comparing the quantity, expansion and characteristics of the helper T cells before and after infection and vaccination.