发明名称 Improved Methods for Inducing Apomixis in Plants
摘要 The present invention relates to methods for inducing apomixis in a plant, methods for the production of apomictic plants and the plants and plant seeds obtained thereby.
申请公布号 US2015299727(A1) 申请公布日期 2015.10.22
申请号 US201314648401 申请日期 2013.11.27
申请人 LEIBNIZ-INSTITUT FÜR PFLANZENGENETIK UND KULTURPFLANZENFORSCHUNG 发明人 Sharbel Timothy;Corral José M.
分类号 C12N15/82;C12N9/22 主分类号 C12N15/82
代理机构 代理人
主权项 1. A method for the production of a transgenic apomictic plant, comprising the following steps: a) providing a plant cell, b) transforming said plant cell with at least one plant vector containing at least one exogenous nucleotide sequence element so as to obtain a transgenic plant cell comprising said at least one exogenous nucleotide sequence element and which transgenic plant cell comprises a nucleotide sequence coding for a trans-acting apomixis effector, a cis-acting regulatory element and a nucleotide sequence coding for a protein with DEDDh exonuclease activity, which is under control of said cis-acting regulatory element, wherein said trans-acting apomixis effector is capable of interacting with said cis-acting regulatory element and wherein said cis-acting regulatory element comprises at least one regulatory nucleotide core sequence selected from the group consisting of an ATHB-5 binding site, a LIM-1 binding site, a SORLIP1AT binding site, a SORLIP2AT binding site and a POLASIG1 binding site, and c) regenerating the transformed plant cell into a transgenic plant exhibiting apomixis.
地址 OT Gatersleben DE