主权项 |
1. A method for the production of a transgenic apomictic plant, comprising the following steps:
a) providing a plant cell, b) transforming said plant cell with at least one plant vector containing at least one exogenous nucleotide sequence element so as to obtain a transgenic plant cell comprising said at least one exogenous nucleotide sequence element and which transgenic plant cell comprises a nucleotide sequence coding for a trans-acting apomixis effector, a cis-acting regulatory element and a nucleotide sequence coding for a protein with DEDDh exonuclease activity, which is under control of said cis-acting regulatory element, wherein said trans-acting apomixis effector is capable of interacting with said cis-acting regulatory element and wherein said cis-acting regulatory element comprises at least one regulatory nucleotide core sequence selected from the group consisting of an ATHB-5 binding site, a LIM-1 binding site, a SORLIP1AT binding site, a SORLIP2AT binding site and a POLASIG1 binding site, and c) regenerating the transformed plant cell into a transgenic plant exhibiting apomixis. |