Method for positioning structures in indentations and arrangements thus obtainable

摘要

Positioning structures in at least one indentation present on a or in a support, wherein said indentation is an indentation having a diameter in the nanometer range, makes it possible to position the structure in the indentation substantially centrally with defined orientation. A support having at least one indentation, wherein this is one having a size in the nanometer range, includes a predetermined structure which is arranged substantially centrally within said indentation and which optionally has a functional unit diametrically opposite to the side pointing to the bottom surface. The arrangement is especially suitable for single molecule analysis and, here especially, for single molecule sequencing and other high-throughput methods.

主权项

1. Method for positioning structures in at least one indentation present on a support and having a diameter in the nanometer range of ≦300 nm, comprising the steps of
a. providing a support having at least one indentation having a size in the nanometer range of 5300 nm, wherein first binding partners are arranged on the bottom surface which is exposed as a result of the indentation on the support and which is preferably planar and permeable to light, b. providing a structure having a predetermined structure formed from nucleic acid, the structure has on one side second binding partners which can form a binding pair with the first binding partners, c. introducing said structure into the at least one indentation and immobilizing said structure in the indentation via the binding partners with the formation of a binding pair between these,wherein the second binding partner is arranged on only one side of the structure in such a way that said structure is positioned substantially centrally in the indentation.