| 摘要 |
The present invention relates to a method for increasing the production of astaxanthin in Haematococcus pluvialis by a two-stage light cultivation process and, more specifically, to a method for increasing the production of astaxanthin in Haematococcus pluvialis cells by inoculating mature spores (cyst) and adding iron ions under high-temperature autotrophic conditions. The two-stage light cultivation process for the production of astaxanthin under autotrophic conditions using the sun during outdoor cultivation comprising inoculating mature spore (cyst) cells and adding iron ions, according to the present invention, can solve the problem that synthesis of astaxanthin is inhibited by excess generation of LROS(O2 -, H2O2) by effectively converting LROS(O2 -, H2O2) that is generated in a large amount under high-temperature conditions to MROS(O2, OH·) and amplifying an intracellular lipid oxidation signal, thereby more economically increasing the production of astaxanthin. Such astaxanthin is useful in a variety of industrial fields as a potent antioxidant. |
