A GENERALIZABLE ASSAY FOR VIRUS CAPSID ASSEMBLY

摘要

Virus capsids protect the viral genome and play roles in its delivery and intracellular transport, making them an attractive target for antiviral therapeutics. The difficulty in targetting capsid assembly is to identify molecules that interfere with the weak protein-protein interactions that drive the reaction. We have developed an in vitro assay for capsid assembly that works on a range of viruses at biologically relevant protein concentrations to facilitate screening large libraries of chemicals for lead compounds.