Quantitative Reverse Transcription Polymerase Chain Reaction Kit for Breast Cancer Drug Screening Test and Early Diagnosis Using Tissue and Blood

摘要

A method for a breast cancer drug screening including separating a full-length RNA from a cell obtained from a tissue or blood of a suspected cancer patient; synthesizing a cDNA from the full-length RNA; performing a PCR with the synthesized cDNA by using a composition including sets of a Pair of primers and a probe, each set independently able to amplify human epidermal growth factor receptor 2 (HER2), cytokeratin 19, EpCAM, a hTERT, Ki67, vimentin, and a GAPDH. The method further includes comparing an amount of the amplification with an expression amount to a normal person. A kit for performing methodology of the invention.

主权项

1. An information offering method for diagnosing breast cancer comprising:
(a) separating a full-length RNA from a cell obtained from a tissue or blood of a suspected cancer patient; (b) synthesizing a cDNA from the separated full-length RNA; (c) performing a real-time PCR with the synthesized cDNA by using a composition consisting of a primer pair and a probe that can amplify human epidermal growth factor receptor 2 (HER2), a primer pair and a probe that can amplify cytokeratin 19, a primer pair and a probe that can amplify epithelial cell adhesion molecule (EpCAM), a primer pair and a probe that can amplify a human telomerase reverse transcriptase (hTERT), a primer pair and a probe that can amplify Ki67, and a primer pair and a probe that can amplify vimentin, and a primer pair and a probe that can amplify a glyceraldehyde-3-phosphate dehydrogenase (GAPDH); and (d) comparing an amount of the amplification with an expression amount to a normal person.