SEPARATION PROCESSES FOR SOY PROTEIN

摘要

The invention provides a process for the separation of soy protein. The process begins with an aqueous extract or solution of soy protein, which is passed through at least one expanded bed absorption (EBA) process. The EBA process comprises contacting the aqueous extract or solution of soy protein with at least one adsorbent resin, said adsorbent resin comprising at least one ligand (L1 or L2), having particular chemical structures. Proteins of interest (e.g. trypsin inhibitor (TI) protein or beta-conglycinin) are isolated by eluting them from said adsorbent resin. The invention also provides various novel protein compositions obtainable via the method of the invention.

主权项

1. A process for the separation of soy protein, said process comprising the steps of:
i. providing an aqueous extract of soy protein or a solution of soy protein, said extract or solution of soy protein comprising at least two types of soy proteins; ii. passing said aqueous extract or solution of soy protein through at least one expanded bed absorption process, wherein said expanded bed absorption process comprises contacting said aqueous extract or solution of soy protein with at least one adsorbent resin which selectively adsorbs at least a first type of soy protein to provide a non-bound protein fraction and a bound protein fraction, said adsorbent resin comprising:
at least one ligand (L1), said at least one ligand (L1) comprising an aromatic or heteroaromatic ring system and one or more acidic groups, orat least one ligand (L2), said at least one ligand (L2) comprising an alkylamine or alkylarylamine, wherein said alkylamine or alkylarylamine moieties in ligands (L2) comprise an amine substituted with one or more groups selected from:
a. an aryl, benzyl or heteroaryl group;b. an alkyl group having 4-16 carbon atoms which may be straight, branched or cyclic;or combinations thereof; iii. isolating said first type of soy protein from said adsorbent resin, by elution of either the non-bound protein fraction or of the bound protein fraction; and iv. isolating the second type of soy protein from said adsorbent resin to provide a second soy protein composition which is depleted in said first type of soy protein.