CIRCULATING BIOMARKERS FOR DISEASE

摘要

Biomarkers can be assessed for diagnostic, therapy-related or prognostic methods to identify phenotypes, such as a condition or disease, or the stage or progression of a disease. Circulating biomarkers from a bodily fluid can be used in profiling of physiological states or determining phenotypes. These include nucleic acids, protein, and circulating structures such as vesicles. Biomarkers can be used for theranostic purposes to select candidate treatment regimens for diseases, conditions, disease stages, and stages of a condition, and can also be used to determine treatment efficacy. The biomarkers can be circulating biomarkers, including vesicles and microRNA.

主权项

1. A method of detecting or grading a cancer in a subject comprising:
(a) isolating a population of microvesicles from a biological sample originating from the subject; (b) contacting the microvesicle population with at least one binding agent that specifically binds to a GPC1 polypeptide or a functional fragment thereof; (c) detecting a presence or level of the at least one binding agent bound to GPC1 associated with the microvesicle population; and (d) comparing the presence or level to a reference to detect or grade the cancer.